Hotspot

rpl33-rps18

LSC · 72057-72407 · 351 bp

Back to Thespesia

Pi 0.0215

MarkerSeek score 69.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 7
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAATCGACACAATACGCCCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	528-586 bp	1.0	81.9
rpl33-rps18_p2	GCCCAGTCGATTGGAATTGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.2	57.7	513-571 bp	1.0	80.8
rpl33-rps18_p3	ACAATACGCCCAGTCGATTG	TCAATTCGATCCCCCGATTG	50.0	50.0	58.3	57.7	520-578 bp	1.0	80.0
rpl33-rps18_p4	CACAATACGCCCAGTCGATT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.3	57.7	521-579 bp	1.0	80.0
rpl33-rps18_p5	AATCGACACAATACGCCCAG	TCAATTCGATCCCCCGATTG	50.0	50.0	58.3	57.7	527-585 bp	1.0	79.9