Hotspot

psaJ-rpl33

LSC · 71348-71855 · 508 bp

Back to Thespesia

Pi 0.0218

MarkerSeek score 70.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 11
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCGTTAACATT	ATTAACGCCGTTTCGAACAC	45.0	45.0	57.9	57.4	613-617 bp	1.0	76.4
psaJ-rpl33_p2	TTCCCGGATGCGTTAACATT	TAACGCCGTTTCGAACACAA	45.0	45.0	57.9	58.7	611-615 bp	1.0	75.3
psaJ-rpl33_p3	TTCCCGGATGCGTTAACATT	TTAACGCCGTTTCGAACACA	45.0	45.0	57.9	58.7	612-616 bp	1.0	75.3
psaJ-rpl33_p4	GTTTCTTCCCGGATGCGTTA	ATTAACGCCGTTTCGAACAC	50.0	45.0	58.3	57.4	618-622 bp	1.0	75.1
psaJ-rpl33_p5	TTCTTCCCGGATGCGTTAAC	ATTAACGCCGTTTCGAACAC	50.0	45.0	58.3	57.4	616-620 bp	1.0	75.1