Hotspot

petG-trnW-CCA

LSC · 70330-70521 · 192 bp

Back to Thespesia

Pi 0.0632

MarkerSeek score 78.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 11
pi: 0.0632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	256-274 bp	1.0	85.8
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	278-296 bp	1.0	85.7
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	276-294 bp	1.0	83.1
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.4	58.0	272-290 bp	1.0	82.5
petG-trnW-CCA_p5	CAGACGTGGTGATCAGTTGG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.6	58.0	277-295 bp	1.0	82.4