Hotspot

petA-psbJ

LSC · 67235-68341 · 1107 bp

Back to Thespesia

Pi 0.0282

MarkerSeek score 63.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 31
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.5	57.6	1167-1180 bp	1.0	75.2
petA-psbJ_p2	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.2	57.6	1166-1179 bp	1.0	72.9
petA-psbJ_p3	GAGAAGGTTCAAGTGTCCGAA	ATTCTGGATTGGGTTCGTCC	47.6	50.0	57.9	57.6	1166-1179 bp	1.0	70.5
petA-psbJ_p4	TTGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	47.6	50.0	58.2	57.6	1168-1181 bp	1.0	70.0
petA-psbJ_p5	AGAAGGTTCAAGTGTCCGAA	ATTCTGGATTGGGTTCGTCC	45.0	50.0	56.7	57.6	1165-1178 bp	1.0	69.0