Hotspot

accD-psaI

LSC · 62513-63259 · 747 bp

Back to Thespesia

Pi 0.0321

MarkerSeek score 72.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 24
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTAGTTCAGCTCCACGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	874-893 bp	1.0	81.9
accD-psaI_p2	AGTTCAGCTCCACGGTTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	869-888 bp	1.0	81.5
accD-psaI_p3	GAGTTAGTTCAGCTCCACGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	880-899 bp	1.0	81.3
accD-psaI_p4	AGTTCAGCTCCACGGTTTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.9	57.9	875-894 bp	1.0	80.9
accD-psaI_p5	GTTAGTTCAGCTCCACGGTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	872-891 bp	1.0	80.6