Hotspot

rpoB-trnC-GCA

LSC · 27699-28925 · 1227 bp

Back to Thespesia

Pi 0.0255
MarkerSeek score 64.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1227
variable_sites
31
pi
0.0255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CTTCTGTTAAGCCCCGATCC CCGGATTTGAACTGGGGAAT 55.0 50.0 58.0 57.9 1390-1393 bp 1.0 81.0
rpoB-trnC-GCA_p2 TTTCCCCATTTTCATCCCCG CCGGATTTGAACTGGGGAAT 50.0 50.0 58.1 57.9 1315-1318 bp 1.0 80.3
rpoB-trnC-GCA_p3 CCGATCCATGAACCCACAAA CCGGATTTGAACTGGGGAAT 50.0 50.0 58.2 57.9 1377-1380 bp 1.0 80.0
rpoB-trnC-GCA_p4 CCATTTTCATCCCCGAGCAT CCGGATTTGAACTGGGGAAT 50.0 50.0 58.3 57.9 1310-1313 bp 1.0 79.0
rpoB-trnC-GCA_p5 TTAAGCCCCGATCCATGAAC CCGGATTTGAACTGGGGAAT 50.0 50.0 57.7 57.9 1384-1387 bp 1.0 78.6