Hotspot

trnR-UCU-atpA

LSC · 10163-10383 · 221 bp

Back to Thespesia

Pi 0.0814

MarkerSeek score 78.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 18
pi: 0.0814
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TCACTGAGGAAGCAGAAACG	47.6	50.0	57.3	57.8	323 bp	1.0	69.7
trnR-UCU-atpA_p2	TGGATAGGACAGAGGTCTTCT	TCACTGAGGAAGCAGAAACG	47.6	50.0	56.9	57.8	358 bp	1.0	66.4
trnR-UCU-atpA_p3	CGTAATGAAAAGCGTCCATTGT	TCACTGAGGAAGCAGAAACG	40.9	50.0	57.9	57.8	384 bp	1.0	65.9
trnR-UCU-atpA_p4	TCGTAATGAAAAGCGTCCATTG	TCACTGAGGAAGCAGAAACG	40.9	50.0	57.7	57.8	385 bp	1.0	63.8
trnR-UCU-atpA_p5	ATGAAAAGCGTCCATTGTCT	TCACTGAGGAAGCAGAAACG	40.0	50.0	55.6	57.8	380 bp	1.0	63.7