Hotspot

rps16-trnQ-UUG

LSC · 6325-6916 · 592 bp

Back to Thespesia

Pi 0.032

MarkerSeek score 71.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 18
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	683-704 bp	1.0	84.8
rps16-trnQ-UUG_p2	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	662-683 bp	1.0	84.8
rps16-trnQ-UUG_p3	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	660-681 bp	1.0	84.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	644-665 bp	1.0	84.0
rps16-trnQ-UUG_p5	AGTGGTGGGTTTTGACATCC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.0	58.2	690-711 bp	1.0	83.6