Hotspot

trnK-UUU-rps16

LSC · 4370-5189 · 820 bp

Back to Thespesia

Pi 0.0318

MarkerSeek score 63.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 26
pi: 0.0318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAGGAGCTCAACCCACAG	55.0	50.0	58.0	58.0	1043-1086 bp	1.0	82.0
trnK-UUU-rps16_p2	AGACAGGCGGATTCATCAAC	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.0	58.0	1559-1609 bp	1.0	81.6
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1182-1225 bp	1.0	81.5
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	1183-1226 bp	1.0	81.5
trnK-UUU-rps16_p5	AGACAGGCGGATTCATCAAC	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	1698-1748 bp	1.0	81.2