Hotspot

ycf1

SSC · 108025-113616 · 5592 bp

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Pi 0.0438

MarkerSeek score 68.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 465
pi: 0.0438
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAACGAGTGCCATTATTGGT	GGCTTTGGCTCTTCTCAGAA	40.0	50.0	55.6	57.8	1027 bp	0.25	29.5
ycf1_p2	AAACGAGTGCCATTATTGGT	AAGGAGTTGTCAGGACAGGT	40.0	50.0	55.6	58.2	830 bp	0.25	29.5
ycf1_p3	AAACGAGTGCCATTATTGGT	AGGAGTTGTCAGGACAGGTA	40.0	50.0	55.6	57.3	829 bp	0.25	25.4
ycf1_p4	TCGATAATTGGGCCAAAGAA	GGCTTTGGCTCTTCTCAGAA	40.0	50.0	55.0	57.8	968 bp	0.25	24.6
ycf1_p5	TCGATAATTGGGCCAAAGAA	AAGGAGTTGTCAGGACAGGT	40.0	50.0	55.0	58.2	771 bp	0.25	24.6