Hotspot

rpl32-trnL-UAG

SSC · 104565-105065 · 501 bp

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Pi 0.0978
MarkerSeek score 80.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
501
variable_sites
85
pi
0.0978
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCGGTGGTTGTGATAGGACT ATCCGCATAAAAGAGCTGCA 50.0 45.0 58.1 58.0 1863-2033 bp 1.0 90.8
rpl32-trnL-UAG_p2 TCGGTGGTTGTGATAGGACT TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.1 58.0 1862-2032 bp 1.0 90.8
rpl32-trnL-UAG_p3 GCGAAATCCCTATCCACTGG ATCCGCATAAAAGAGCTGCA 55.0 45.0 58.1 58.0 1174-1195 bp 1.0 90.5
rpl32-trnL-UAG_p4 GCGAAATCCCTATCCACTGG TCCGCATAAAAGAGCTGCAT 55.0 45.0 58.1 58.0 1173-1194 bp 1.0 90.5
rpl32-trnL-UAG_p5 TCGGTGGTTGTGATAGGACT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1260-1430 bp 1.0 90.5