Hotspot

ycf1-rpl32

SSC · 104300-104411 · 112 bp

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Pi 0.11

MarkerSeek score 77.40

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 112
variable_sites: 20
pi: 0.11
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	TCGGTGGTTGTGATAGGACT	TTCACCATGGCAGCCTATTC	50.0	50.0	58.1	57.9	1235-1405 bp	1.0	91.0
ycf1-rpl32_p2	TCGGTGGTTGTGATAGGACT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1260-1430 bp	1.0	90.5
ycf1-rpl32_p3	TCGGTGGTTGTGATAGGACT	GAATCCCCATCCCGGAAAAA	50.0	50.0	58.1	57.8	917-1085 bp	1.0	90.2
ycf1-rpl32_p4	TCGGTGGTTGTGATAGGACT	CCAGTGGATAGGGATTTCGC	50.0	55.0	58.1	58.1	689-879 bp	1.0	88.8
ycf1-rpl32_p5	CTCTGTTCCACGGCTCATTT	TTCACCATGGCAGCCTATTC	50.0	50.0	58.2	57.9	1514-1684 bp	0.75	79.2