Hotspot

ycf4-cemA

LSC · 57440-58129 · 690 bp

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Pi 0.0529

MarkerSeek score 83.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 70
pi: 0.0529
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGGGGATTTCCTGGAAA	GGCGAATGGAATCCGATACA	50.0	50.0	58.1	57.8	1436-1448 bp	1.0	84.6
ycf4-cemA_p2	TTTCGTTGGGGATTTCCTGG	GGCGAATGGAATCCGATACA	50.0	50.0	58.1	57.8	1439-1451 bp	1.0	84.6
ycf4-cemA_p3	TGAATTGGCGATCAGAACGT	GGCGAATGGAATCCGATACA	45.0	50.0	57.9	57.8	1750-1762 bp	1.0	83.8
ycf4-cemA_p4	CGTTGGGGATTTCCTGGAAA	CGTCCAACAGAAGGAGTTCC	50.0	55.0	58.1	58.2	1147-1158 bp	0.75	70.4
ycf4-cemA_p5	TTTCGTTGGGGATTTCCTGG	CGTCCAACAGAAGGAGTTCC	50.0	55.0	58.1	58.2	1150-1161 bp	0.75	70.3