Hotspot

trnG-GCC-trnM-CAU

LSC · 35934-36114 · 181 bp

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Pi 0.0635

MarkerSeek score 77.20

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 18
pi: 0.0635
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	250-260 bp	1.0	88.3
trnG-GCC-trnM-CAU_p2	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	279-289 bp	1.0	87.9
trnG-GCC-trnM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	248-258 bp	1.0	87.2
trnG-GCC-trnM-CAU_p4	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.9	57.8	277-287 bp	1.0	86.8
trnG-GCC-trnM-CAU_p5	ATGCGGATATGGTCGAATGG	GCAGCTCGTAAGGCTCATAA	50.0	50.0	57.9	57.8	300-310 bp	1.0	85.8