Hotspot

trnD-GUC-trnY-GUA

LSC · 29986-30399 · 414 bp

Back to Thesium

Pi 0.0396

MarkerSeek score 77.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 31
pi: 0.0396
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	50.0	50.0	58.5	58.0	515-566 bp	1.0	84.4
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GCAATACGTCTACGCTGGTT	50.0	50.0	58.5	58.4	460-511 bp	1.0	82.4
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GTTGGCAATACGTCTACGCT	50.0	50.0	58.5	58.4	464-515 bp	1.0	82.4
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	TTGGCAATACGTCTACGCTG	50.0	50.0	58.5	58.4	463-514 bp	1.0	82.4
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	483-534 bp	1.0	82.3