Hotspot

petN-psbM

LSC · 27957-28737 · 781 bp

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Pi 0.0578

MarkerSeek score 82.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 70
pi: 0.0578
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTTCGAAGGATGTCACTGC	TTTTATGGGTGCGTTCCACT	50.0	45.0	57.9	57.7	1779-1804 bp	0.75	76.3
petN-psbM_p2	ACTTCGAAGGATGTCACTGC	TTATGGGTGCGTTCCACTAC	50.0	50.0	57.9	57.6	1777-1802 bp	0.75	75.4
petN-psbM_p3	GTTGTGAAAAGGGCGGGATA	TTTTATGGGTGCGTTCCACT	50.0	45.0	58.2	57.7	2168-2178 bp	0.75	70.7
petN-psbM_p4	AGTAGTTGTGAAAAGGGCGG	TTTTATGGGTGCGTTCCACT	50.0	45.0	58.1	57.7	2182 bp	0.25	46.9
petN-psbM_p5	AGTAGTTGTGAAAAGGGCGG	TTATGGGTGCGTTCCACTAC	50.0	50.0	58.1	57.6	2180 bp	0.25	46.0