Hotspot

trnR-UCU-atpA

LSC · 9855-10053 · 199 bp

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Pi 0.0511

MarkerSeek score 77.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 18
pi: 0.0511
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATTCCCGCTATCCGCTTTAC	AGACATTCACGGAAGAAGCC	50.0	50.0	57.8	57.9	687-716 bp	1.0	89.9
trnR-UCU-atpA_p2	ATTCCCGCTATCCGCTTTAC	GGAAGAAGCCGAATCCCTTT	50.0	50.0	57.8	57.9	677-706 bp	1.0	89.6
trnR-UCU-atpA_p3	ATTCCCGCTATCCGCTTTAC	ATTGGAACTGGCGCAATTTG	50.0	45.0	57.8	57.9	974-1003 bp	1.0	88.7
trnR-UCU-atpA_p4	ATTCCCGCTATCCGCTTTAC	ATGAAACAAGTAGCCGGCAA	50.0	45.0	57.8	58.1	1000-1029 bp	1.0	88.3
trnR-UCU-atpA_p5	ATTCCCGCTATCCGCTTTAC	TCAAGAGCAAATGGAACGCT	50.0	45.0	57.8	58.1	648-673 bp	0.75	79.6