Hotspot

psbK-psbI

LSC · 7692-8132 · 441 bp

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Pi 0.0493

MarkerSeek score 79.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 40
pi: 0.0493
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TCAAAACCCAGTGCCTTACC	TGGACTAAAGCGGTGGATTG	50.0	50.0	58.0	57.9	1219-1242 bp	0.75	71.7
psbK-psbI_p2	TCAAAACCCAGTGCCTTACC	GTGGACTAAAGCGGTGGATT	50.0	50.0	58.0	57.9	1220-1243 bp	0.75	71.6
psbK-psbI_p3	GCCAAATTACCCGAAGCCTA	TAGCCGGCCTGTATTAGTGA	50.0	50.0	58.0	57.9	918-926 bp	0.5	62.7
psbK-psbI_p4	TCAAAACCCAGTGCCTTACC	TAGCCGGCCTGTATTAGTGA	50.0	50.0	58.0	57.9	1376-1397 bp	0.5	62.1
psbK-psbI_p5	CTCCGAATGCTGGGATCAAA	TAGCCGGCCTGTATTAGTGA	50.0	50.0	58.0	57.9	1391-1412 bp	0.5	61.8