Hotspot

trnH-GUG-psbA

LSC · 92-549 · 458 bp

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Pi 0.0938

MarkerSeek score 81.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 76
pi: 0.0938
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGTAGCTGCCCATGGTTATT	55.0	50.0	58.1	58.0	790-828 bp	1.0	90.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TGCATGGTTCCTTGGTAACC	55.0	50.0	58.1	58.1	893-931 bp	1.0	88.8
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGGAATGGGAGCTTAGCTTC	55.0	55.0	58.1	58.0	1148-1186 bp	1.0	87.4
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	902-940 bp	0.75	73.4
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	901-939 bp	0.75	73.4