primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CGTAGCTGCCCATGGTTATT	20	20	55.0	50.0	58.1	58.0	0.0	11.7	0.161601	92	549	790-828	1.000000	90.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TGCATGGTTCCTTGGTAACC	20	20	55.0	50.0	58.1	58.1	0.0	11.5	0.211048	92	549	893-931	1.000000	88.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	GGGAATGGGAGCTTAGCTTC	20	20	55.0	55.0	58.1	58.0	0.0	8.6	0.154655	92	549	1148-1186	1.000000	87.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	92	549	902-940	0.750000	73.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	92	549	901-939	0.750000	73.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AATCCTGTCCTTCAAGTCGC	GTTCGAATCCTTCCGTTCCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.189554	5948	7080	1186-1401	1.000000	89.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GTTCGAATCCTTCCGTTCCA	20	20	45.0	50.0	58.1	57.9	8.1	0.0	0.228274	5948	7080	1173-1388	1.000000	89.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCTGTCCTTCAAGTCGC	TTCGAATCCTTCCGTTCCAG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.480688	5948	7080	1185-1400	1.000000	87.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AATCCTGTCCTTCAAGTCGC	AGGTTCGAATCCTTCCGTTC	20	20	50.0	50.0	57.9	57.6	0.0	4.4	0.482329	5948	7080	1188-1403	1.000000	87.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AATCCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTT	20	20	50.0	50.0	57.9	57.6	0.0	16.9	0.482329	5948	7080	1189-1404	1.000000	87.2
psbK-psbI_p1	psbK-psbI	1	TCAAAACCCAGTGCCTTACC	TGGACTAAAGCGGTGGATTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.118244	7692	8132	1219-1242	0.750000	71.7
psbK-psbI_p2	psbK-psbI	2	TCAAAACCCAGTGCCTTACC	GTGGACTAAAGCGGTGGATT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119718	7692	8132	1220-1243	0.750000	71.6
psbK-psbI_p3	psbK-psbI	3	GCCAAATTACCCGAAGCCTA	TAGCCGGCCTGTATTAGTGA	20	20	50.0	50.0	58.0	57.9	0.0	26.1	0.123209	7692	8132	918-926	0.500000	62.7
psbK-psbI_p4	psbK-psbI	4	TCAAAACCCAGTGCCTTACC	TAGCCGGCCTGTATTAGTGA	20	20	50.0	50.0	58.0	57.9	0.0	26.1	0.086501	7692	8132	1376-1397	0.500000	62.1
psbK-psbI_p5	psbK-psbI	5	CTCCGAATGCTGGGATCAAA	TAGCCGGCCTGTATTAGTGA	20	20	50.0	50.0	58.0	57.9	0.0	26.1	0.118893	7692	8132	1391-1412	0.500000	61.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCACCGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	0.0	0.0	3.643792	8362	8713	404-679	1.000000	61.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCACCGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	0.0	0.0	3.645267	8362	8713	403-678	1.000000	61.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GCAGAAAGGGAAAGAGAGGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	57.9	55.5	0.0	0.0	3.657134	8362	8713	464-739	1.000000	60.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CTCGTACAGCGGATTAGCAA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.739643	8362	8713	421-696	1.000000	60.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AACTCGTACAGCGGATTAGC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.741149	8362	8713	423-698	1.000000	60.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	TTCCAAGCTAATGATGCGGG	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.3	54.6	0.0	0.0	4.725360	9464	9782	377-420	1.000000	54.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	GCTAATGATGCGGGTTCGAT	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.5	54.6	0.0	0.0	4.891573	9464	9782	371-414	1.000000	52.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	CCAAGCTAATGATGCGGGTT	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.6	54.6	0.0	0.0	5.011909	9464	9782	375-418	1.000000	52.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	AATGATGCGGGTTCGATTCC	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.7	54.6	0.0	0.0	5.095501	9464	9782	368-411	1.000000	52.0
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AAGCTAATGATGCGGGTTCG	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.7	54.6	0.0	0.0	5.104958	9464	9782	373-416	1.000000	52.0
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	ATTCCCGCTATCCGCTTTAC	AGACATTCACGGAAGAAGCC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.265923	9855	10053	687-716	1.000000	89.9
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	ATTCCCGCTATCCGCTTTAC	GGAAGAAGCCGAATCCCTTT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.295591	9855	10053	677-706	1.000000	89.6
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	ATTCCCGCTATCCGCTTTAC	ATTGGAACTGGCGCAATTTG	20	20	50.0	45.0	57.8	57.9	0.0	10.8	0.248485	9855	10053	974-1003	1.000000	88.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	ATTCCCGCTATCCGCTTTAC	ATGAAACAAGTAGCCGGCAA	20	20	50.0	45.0	57.8	58.1	0.0	31.5	0.261032	9855	10053	1000-1029	1.000000	88.3
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	ATTCCCGCTATCCGCTTTAC	TCAAGAGCAAATGGAACGCT	20	20	50.0	45.0	57.8	58.1	0.0	0.0	0.269099	9855	10053	648-673	0.750000	79.6
petN-psbM_p1	petN-psbM	1	ACTTCGAAGGATGTCACTGC	TTTTATGGGTGCGTTCCACT	20	20	50.0	45.0	57.9	57.7	27.9	0.0	0.365989	27957	28737	1779-1804	0.750000	76.3
petN-psbM_p2	petN-psbM	2	ACTTCGAAGGATGTCACTGC	TTATGGGTGCGTTCCACTAC	20	20	50.0	50.0	57.9	57.6	27.9	0.0	0.476016	27957	28737	1777-1802	0.750000	75.4
petN-psbM_p3	petN-psbM	3	GTTGTGAAAAGGGCGGGATA	TTTTATGGGTGCGTTCCACT	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.450955	27957	28737	2168-2178	0.750000	70.7
petN-psbM_p4	petN-psbM	4	AGTAGTTGTGAAAAGGGCGG	TTTTATGGGTGCGTTCCACT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.387725	27957	28737	2182-2182	0.250000	46.9
petN-psbM_p5	petN-psbM	5	AGTAGTTGTGAAAAGGGCGG	TTATGGGTGCGTTCCACTAC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.497752	27957	28737	2180-2180	0.250000	46.0
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	20	20	50.0	50.0	58.5	58.0	7.1	0.0	0.521454	29986	30399	515-566	1.000000	84.4
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GCAATACGTCTACGCTGGTT	20	20	50.0	50.0	58.5	58.4	7.1	1.7	0.840344	29986	30399	460-511	1.000000	82.4
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GTTGGCAATACGTCTACGCT	20	20	50.0	50.0	58.5	58.4	7.1	1.9	0.840344	29986	30399	464-515	1.000000	82.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	TTGGCAATACGTCTACGCTG	20	20	50.0	50.0	58.5	58.4	7.1	1.7	0.841487	29986	30399	463-514	1.000000	82.4
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	29986	30399	483-534	1.000000	82.3
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	35934	36114	250-260	1.000000	88.3
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	4.2	0.137348	35934	36114	279-289	1.000000	87.9
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	35934	36114	248-258	1.000000	87.2
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.278053	35934	36114	277-287	1.000000	86.8
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	ATGCGGATATGGTCGAATGG	GCAGCTCGTAAGGCTCATAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.324209	35934	36114	300-310	1.000000	85.8
ycf4-cemA_p1	ycf4-cemA	1	CGTTGGGGATTTCCTGGAAA	GGCGAATGGAATCCGATACA	20	20	50.0	50.0	58.1	57.8	23.1	0.0	0.245946	57440	58129	1436-1448	1.000000	84.6
ycf4-cemA_p2	ycf4-cemA	2	TTTCGTTGGGGATTTCCTGG	GGCGAATGGAATCCGATACA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.245946	57440	58129	1439-1451	1.000000	84.6
ycf4-cemA_p3	ycf4-cemA	3	TGAATTGGCGATCAGAACGT	GGCGAATGGAATCCGATACA	20	20	45.0	50.0	57.9	57.8	1.7	0.0	0.257124	57440	58129	1750-1762	1.000000	83.8
ycf4-cemA_p4	ycf4-cemA	4	CGTTGGGGATTTCCTGGAAA	CGTCCAACAGAAGGAGTTCC	20	20	50.0	55.0	58.1	58.2	23.1	0.0	0.285103	57440	58129	1147-1158	0.750000	70.4
ycf4-cemA_p5	ycf4-cemA	5	TTTCGTTGGGGATTTCCTGG	CGTCCAACAGAAGGAGTTCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.285103	57440	58129	1150-1161	0.750000	70.3
petA-psbJ_p1	petA-psbJ	1	ATTCTTTCCCCGGACCCTTA	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.029153	60022	60500	1530-1549	1.000000	84.3
petA-psbJ_p2	petA-psbJ	2	TATTCTTTCCCCGGACCCTT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.029153	60022	60500	1531-1550	1.000000	84.3
petA-psbJ_p3	petA-psbJ	3	TTCTTTCCCCGGACCCTTAT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.029153	60022	60500	1529-1548	1.000000	84.3
petA-psbJ_p4	petA-psbJ	4	ATTCTTTCCCCGGACCCTTA	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.029153	60022	60500	1535-1550	0.750000	71.3
petA-psbJ_p5	petA-psbJ	5	TTCTTTCCCCGGACCCTTAT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.7	0.0	0.029153	60022	60500	1534-1549	0.750000	71.3
rpl16-rps3_p1	rpl16-rps3	1	AACGAGTCACACACTAAGCA	TGCTCCTATCCAATTCGAACT	20	21	45.0	42.9	57.1	56.8	0.0	0.0	3.075858	78758	78938	268-283	1.000000	63.3
rpl16-rps3_p2	rpl16-rps3	2	ACGAGTCACACACTAAGCAT	TGCTCCTATCCAATTCGAACT	20	21	45.0	42.9	56.9	56.8	0.0	0.0	3.302146	78758	78938	267-282	1.000000	61.5
rpl16-rps3_p3	rpl16-rps3	3	AACGAGTCACACACTAAGCA	TTGCTCCTATCCAATTCGAACT	20	22	45.0	40.9	57.1	57.5	0.0	0.0	3.370642	78758	78938	269-284	1.000000	60.9
rpl16-rps3_p4	rpl16-rps3	4	AACGAGTCACACACTAAGCAT	TGCTCCTATCCAATTCGAACT	21	21	42.9	42.9	57.6	56.8	0.0	0.0	3.579700	78758	78938	268-283	1.000000	59.2
rpl16-rps3_p5	rpl16-rps3	5	ACGAGTCACACACTAAGCAT	TTGCTCCTATCCAATTCGAACT	20	22	45.0	40.9	56.9	57.5	0.0	0.0	3.596929	78758	78938	268-283	1.000000	59.1
ycf1-rpl32_p1	ycf1-rpl32	1	TCGGTGGTTGTGATAGGACT	TTCACCATGGCAGCCTATTC	20	20	50.0	50.0	58.1	57.9	0.0	13.0	0.124116	104300	104411	1235-1405	1.000000	91.0
ycf1-rpl32_p2	ycf1-rpl32	2	TCGGTGGTTGTGATAGGACT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.191256	104300	104411	1260-1430	1.000000	90.5
ycf1-rpl32_p3	ycf1-rpl32	3	TCGGTGGTTGTGATAGGACT	GAATCCCCATCCCGGAAAAA	20	20	50.0	50.0	58.1	57.8	0.0	0.7	0.220012	104300	104411	917-1085	1.000000	90.2
ycf1-rpl32_p4	ycf1-rpl32	4	TCGGTGGTTGTGATAGGACT	CCAGTGGATAGGGATTTCGC	20	20	50.0	55.0	58.1	58.1	0.0	1.7	0.182743	104300	104411	689-879	1.000000	88.8
ycf1-rpl32_p5	ycf1-rpl32	5	CTCTGTTCCACGGCTCATTT	TTCACCATGGCAGCCTATTC	20	20	50.0	50.0	58.2	57.9	0.0	13.0	0.243624	104300	104411	1514-1684	0.750000	79.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCGGTGGTTGTGATAGGACT	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.1	58.0	0.0	1.0	0.109665	104565	105065	1863-2033	1.000000	90.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCGGTGGTTGTGATAGGACT	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.1	58.0	0.0	1.0	0.109665	104565	105065	1862-2032	1.000000	90.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGAAATCCCTATCCACTGG	ATCCGCATAAAAGAGCTGCA	20	20	55.0	45.0	58.1	58.0	0.0	1.0	0.158451	104565	105065	1174-1195	1.000000	90.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGAAATCCCTATCCACTGG	TCCGCATAAAAGAGCTGCAT	20	20	55.0	45.0	58.1	58.0	0.0	1.0	0.158451	104565	105065	1173-1194	1.000000	90.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCGGTGGTTGTGATAGGACT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.191256	104565	105065	1260-1430	1.000000	90.5
psaC-rps15_p1	psaC-rps15	1	CATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	24	20	29.2	40.0	53.4	53.0	0.0	10.8	13.576719	106780	107412	681-723	1.000000	51.4
psaC-rps15_p2	psaC-rps15	2	TCATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	25	20	28.0	40.0	54.8	53.0	0.0	10.8	13.197793	106780	107412	682-724	1.000000	51.3
psaC-rps15_p3	psaC-rps15	3	ATCATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	26	20	26.9	40.0	55.3	53.0	0.0	10.8	13.704260	106780	107412	683-725	1.000000	51.3
psaC-rps15_p4	psaC-rps15	4	TCATAAATCTTTACTGAATGTGACAT	TCAGTTGAATATTCGGGAAC	26	20	26.9	40.0	55.3	53.0	0.0	10.8	13.704260	106780	107412	682-724	1.000000	51.3
psaC-rps15_p5	psaC-rps15	5	TCATAAATCTTTACTGAATGTGAC	TCAGTTGAATATTCGGGAAC	24	20	29.2	40.0	53.2	53.0	0.0	10.8	13.823613	106780	107412	682-724	1.000000	51.3
rps15-ycf1_p1	rps15-ycf1	1	TGAAATTCGACTGATCCCCG	TTCTTTGGCCCAATTATCGA	20	20	50.0	40.0	57.7	55.0	0.0	0.0	3.297892	107686	108024	538-561	1.000000	62.1
rps15-ycf1_p2	rps15-ycf1	2	TGAAATTCGACTGATCCCCG	TCGAACCAATTTGTTTGTCTGT	20	22	50.0	36.4	57.7	57.0	0.0	9.0	3.283896	107686	108024	907-916	0.750000	46.9
rps15-ycf1_p3	rps15-ycf1	3	TGAAATTCGACTGATCCCCG	CAGCAAATGAATCAAAGCTACCA	20	23	50.0	39.1	57.7	57.9	0.0	7.0	3.372708	107686	108024	821-824	0.750000	46.2
rps15-ycf1_p4	rps15-ycf1	4	TGAAATTCGACTGATCCCCG	ACAGCAAATGAATCAAAGCTACC	20	23	50.0	39.1	57.7	57.9	0.0	7.0	3.373969	107686	108024	822-825	0.750000	46.2
rps15-ycf1_p5	rps15-ycf1	5	GAAATTCGACTGATCCCCGG	TCGAACCAATTTGTTTGTCTGT	20	22	55.0	36.4	58.4	57.0	0.0	9.0	3.391315	107686	108024	906-915	0.750000	46.0
ycf1_p1	ycf1	1	AAACGAGTGCCATTATTGGT	GGCTTTGGCTCTTCTCAGAA	20	20	40.0	50.0	55.6	57.8	5.6	0.0	2.563439	108341	108460	1027-1027	0.250000	29.5
ycf1_p2	ycf1	2	AAACGAGTGCCATTATTGGT	AAGGAGTTGTCAGGACAGGT	20	20	40.0	50.0	55.6	58.2	5.6	2.0	2.564497	108341	108460	830-830	0.250000	29.5
ycf1_p3	ycf1	3	AAACGAGTGCCATTATTGGT	AGGAGTTGTCAGGACAGGTA	20	20	40.0	50.0	55.6	57.3	5.6	2.0	3.068837	108341	108460	829-829	0.250000	25.4
ycf1_p4	ycf1	4	TCGATAATTGGGCCAAAGAA	GGCTTTGGCTCTTCTCAGAA	20	20	40.0	50.0	55.0	57.8	25.0	0.0	3.179491	108341	108460	968-968	0.250000	24.6
ycf1_p5	ycf1	5	TCGATAATTGGGCCAAAGAA	AAGGAGTTGTCAGGACAGGT	20	20	40.0	50.0	55.0	58.2	25.0	2.0	3.180550	108341	108460	771-771	0.250000	24.6