Hotspot

ycf1

SSC · 116682-121205 · 4524 bp

Back to Thepparatia

Pi 0.00155

MarkerSeek score 54.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4524
variable_sites: 7
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGATATTGAGTCCTGGGTCG	CTGGGAAGAACCAAAGCTCC	55.0	55.0	57.6	58.5	227 bp	1.0	74.0
ycf1_p2	CGATATTGAGTCCTGGGTCG	GGGAAGAACCAAAGCTCCAG	55.0	55.0	57.6	58.5	225 bp	1.0	74.0
ycf1_p3	TTGAGTCCTGGGTCGATACC	GGGAAGAACCAAAGCTCCAG	55.0	55.0	58.5	58.5	220 bp	1.0	73.3
ycf1_p4	TTGAGTCCTGGGTCGATACC	CTGGGAAGAACCAAAGCTCC	55.0	55.0	58.5	58.5	222 bp	1.0	73.2
ycf1_p5	CGATATTGAGTCCTGGGTCG	GGAAGAACCAAAGCTCCAGA	55.0	50.0	57.6	57.4	224 bp	1.0	73.1