Hotspot

trnQ-UUG

LSC · 7240-7311 · 72 bp

Back to Thepparatia

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label trnQ-UUG

Feature Metadata

label_name: trnQ-UUG
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG_p1	AGCAAGAATCACACTTTCTCCA	TGGAACTGGAATTGACGAACA	40.9	42.9	57.9	57.8	254 bp	1.0	54.0
trnQ-UUG_p2	AGCAAGAATCACACTTTCTCCA	ACTGGAATTGACGAACAAGGA	40.9	42.9	57.9	57.5	250 bp	1.0	51.7
trnQ-UUG_p3	AGCAAGAATCACACTTTCTCCA	TCTCTATCCATACGTGCTCG	40.9	50.0	57.9	56.4	305 bp	1.0	50.4
trnQ-UUG_p4	GCAAGAATCACACTTTCTCCA	TGGAACTGGAATTGACGAACA	42.9	42.9	56.5	57.8	253 bp	1.0	50.3
trnQ-UUG_p5	AGCAAGAATCACACTTTCTCCA	TTCTCTATCCATACGTGCTCG	40.9	47.6	57.9	57.1	306 bp	1.0	48.3