Hotspot

rpl32-ndhF

SSC · 132356-133304 · 949 bp

Back to Thepparatia

Pi 0.00212

MarkerSeek score 60.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 949
variable_sites: 2
pi: 0.00212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGAAAAAGCTTTCAACGCCG	TGTTGCCGTCGGAAAAAGTA	45.0	45.0	58.2	58.0	1144-1147 bp	1.0	78.7
rpl32-ndhF_p2	GCCGCCCAATATCCTTTCTT	TGTTGCCGTCGGAAAAAGTA	50.0	45.0	58.3	58.0	1128-1131 bp	1.0	77.6
rpl32-ndhF_p3	TGAAAAAGCTTTCAACGCCG	TTTGTTGCCGTCGGAAAAAG	45.0	45.0	58.2	57.8	1146-1149 bp	1.0	77.5
rpl32-ndhF_p4	GCCGCCCAATATCCTTTCTT	TTTGTTGCCGTCGGAAAAAG	50.0	45.0	58.3	57.8	1130-1133 bp	1.0	76.4
rpl32-ndhF_p5	TGAAAAAGCTTTCAACGCCG	CGGAAGTGGCACAAAAGGTA	45.0	50.0	58.2	58.4	1096-1099 bp	1.0	75.9