Hotspot

atpB-rbcL

LSC · 58715-59958 · 1244 bp

Back to Thepparatia

Pi 0.00166

MarkerSeek score 62.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 2
pi: 0.00166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	ACTCTTTAACACCGGCTTGG	45.0	50.0	58.0	58.1	1351-1405 bp	1.0	81.2
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TTAACACCGGCTTGGAATCC	45.0	50.0	58.0	58.2	1346-1400 bp	1.0	80.7
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	CTCTTTAACACCGGCTTGGA	45.0	50.0	58.0	57.8	1350-1404 bp	1.0	80.6
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	GCTTGGAATCCAACACCTGT	45.0	50.0	58.0	58.4	1337-1391 bp	1.0	79.1
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	TCTTTAACACCGGCTTGGAA	45.0	45.0	58.0	57.4	1349-1403 bp	1.0	76.9