Hotspot

ndhC-trnM-CAU

LSC · 54189-56508 · 2320 bp

Back to Thepparatia

Pi 0.00228

MarkerSeek score 48.10

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 2320
variable_sites: 4
pi: 0.00228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	ACCCTTTCTAATCGGGGCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	1906-2475 bp	1.0	79.4
ndhC-trnM-CAU_p2	CGGGGCTAAAACTCCAGAAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.8	58.4	1894-2463 bp	1.0	79.3
ndhC-trnM-CAU_p3	ATCGGGGCTAAAACTCCAGA	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.4	58.4	1896-2465 bp	1.0	77.5
ndhC-trnM-CAU_p4	AATCGGGGCTAAAACTCCAG	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.6	58.4	1897-2466 bp	1.0	77.5
ndhC-trnM-CAU_p5	ACCCTTTCTAATCGGGGCTA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.8	57.3	1905-2474 bp	1.0	77.4