Hotspot

psaA-ycf3

LSC · 44672-45654 · 983 bp

Back to Thepparatia

Pi 0.00205

MarkerSeek score 60.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 2
pi: 0.00205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTAAAGATCACGAGGCG	50.0	50.0	57.9	58.0	1093-1129 bp	1.0	79.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGTTAAAGATCACGAGGCGT	50.0	50.0	57.9	58.0	1092-1128 bp	1.0	79.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1161-1197 bp	1.0	79.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	1143-1179 bp	1.0	79.4
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TGGTTAAAGATCACGAGGCG	50.0	50.0	58.2	58.0	1116-1152 bp	1.0	79.2