Hotspot

trnS-GCU-trnS-CGA

LSC · 8569-9483 · 915 bp

Back to Thepparatia

Pi 0.00111

MarkerSeek score 60.50

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	1019-1030 bp	1.0	77.5
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	50.0	50.0	58.0	57.6	1018-1029 bp	1.0	77.5
trnS-GCU-trnS-CGA_p3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	50.0	50.0	57.9	57.6	1034-1045 bp	1.0	76.8
trnS-GCU-trnS-CGA_p4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	50.0	50.0	58.2	57.6	1073-1084 bp	1.0	76.3
trnS-GCU-trnS-CGA_p5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	45.0	50.0	58.3	57.6	1035-1046 bp	1.0	75.4