Hotspot

ndhD

SSC · 120771-121922 · 1152 bp

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Pi 0.00463

MarkerSeek score 61.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1152
variable_sites: 8
pi: 0.00463
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTTTTCAATTGTACAACGAACA	TTTAGCTACTTTGGCGGCTT	28.0	45.0	56.7	57.8	1293 bp	1.0	40.5
ndhD_p2	TCATTTTTCAATTGTACAACGAACA	GCCAGTTACTCGAGATTCCC	28.0	55.0	56.7	57.8	1272 bp	1.0	40.5
ndhD_p3	TCATTTTTCAATTGTACAACGAACA	GGCCAGTTACTCGAGATTCC	28.0	55.0	56.7	57.8	1273 bp	1.0	40.5
ndhD_p4	TCATTTTTCAATTGTACAACGAACA	CTTTAGCTACTTTGGCGGCT	28.0	50.0	56.7	58.3	1294 bp	1.0	40.5
ndhD_p5	TTTCAATTGTACAACGAACAA	TTTAGCTACTTTGGCGGCTT	28.6	45.0	52.5	57.8	1288 bp	1.0	40.5