Hotspot

clpP

LSC · 74695-76823 · 2129 bp

Back to Theobroma

Pi 0.00613
MarkerSeek score 48.00
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2129
variable_sites
19
pi
0.00613
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CCCCTGATCTGAACAAGTCG CAAGCGACCAGGATCATTCA 55.0 50.0 58.0 58.0 2941-2948 bp 1.0 80.5
clpP_p2 TCTTTTCTCTCAGCCCCTGA CAAGCGACCAGGATCATTCA 50.0 50.0 58.0 58.0 2954-2961 bp 1.0 80.4
clpP_p3 CCCCTGATCTGAACAAGTCG ATGCTATGGACCTCCCCTTT 55.0 50.0 58.0 58.1 2827-2834 bp 1.0 80.0
clpP_p4 CCTGATCTGAACAAGTCGCA CAAGCGACCAGGATCATTCA 50.0 50.0 57.9 58.0 2939-2946 bp 1.0 79.9
clpP_p5 TCTTTTCTCTCAGCCCCTGA ATGCTATGGACCTCCCCTTT 50.0 50.0 58.0 58.1 2840-2847 bp 1.0 79.9