Hotspot

rrn23S-trnA-TGC

IRa · 139560-139731 · 172 bp

Back to Theobroma

Pi 0.00388
MarkerSeek score 60.60
Primer available yes
Label rrn23S-trnA-TGC

Feature Metadata

label_name
rrn23S-trnA-TGC
feature_type
igs
region
IRa
length_bp
172
variable_sites
1
pi
0.00388
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23S-trnA-TGC_p1 GTCTCTGGGTGCCTAGGTAT GGTTCGAGTCCGCTTATCTC 55.0 55.0 57.9 57.9 230-280 bp 1.0 81.8
rrn23S-trnA-TGC_p2 GTCTCTGGGTGCCTAGGTAT GTTCGAGTCCGCTTATCTCC 55.0 55.0 57.9 57.9 229-279 bp 1.0 81.8
rrn23S-trnA-TGC_p3 ATTCGGGAATCTCCGGATCT GGTTCGAGTCCGCTTATCTC 50.0 55.0 58.0 57.9 302-352 bp 1.0 81.6
rrn23S-trnA-TGC_p4 ATTCGGGAATCTCCGGATCT GTTCGAGTCCGCTTATCTCC 50.0 55.0 58.0 57.9 301-351 bp 1.0 81.6
rrn23S-trnA-TGC_p5 ATTCGGGAATCTCCGGATCT TTCGAGTCCGCTTATCTCCA 50.0 50.0 58.0 58.2 300-350 bp 1.0 80.7