Hotspot

rpl32-trnL-TAG

SSC · 118144-119301 · 1158 bp

Back to Theobroma

Pi 0.00922
MarkerSeek score 50.30
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
1158
variable_sites
16
pi
0.00922
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 TCTCTCTTCGAGCTTGACCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1793-1800 bp 1.0 81.5
rpl32-trnL-TAG_p2 TCTCTCTTCGAGCTTGACCA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1794-1801 bp 1.0 81.5
rpl32-trnL-TAG_p3 CGGCGTTGAAAGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1264-1285 bp 1.0 79.9
rpl32-trnL-TAG_p4 CGGCGTTGAAAGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1265-1286 bp 1.0 79.9
rpl32-trnL-TAG_p5 TTCGAGCTTGACCAATTCCC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 1787 bp 0.333333 51.8