Hotspot

ndhF-rpl32

SSC · 116910-117987 · 1078 bp

Back to Theobroma

Pi 0.0183

MarkerSeek score 63.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1078
variable_sites: 29
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	TGAAAAAGCTTTCAACGCCG	50.0	45.0	58.0	58.2	1211-1228 bp	1.0	81.9
ndhF-rpl32_p2	AAATAGGAACGGGAAGTGGC	TGAAAAAGCTTTCAACGCCG	50.0	45.0	57.9	58.2	1221-1238 bp	1.0	81.0
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	GCCGCCCAATATCCTTTCTT	50.0	50.0	58.0	58.3	1195-1212 bp	1.0	80.9
ndhF-rpl32_p4	AAATAGGAACGGGAAGTGGC	GCCGCCCAATATCCTTTCTT	50.0	50.0	57.9	58.3	1205-1222 bp	1.0	79.9
ndhF-rpl32_p5	GGGAAGTGGCACAAAAGGTA	TTTCAACGCCGCCCAATAT	50.0	47.4	58.0	58.1	1202-1219 bp	1.0	74.3