Hotspot

trnR-ACG-trnN-GTT

IRb · 113704-114300 · 597 bp

Back to Theobroma

Pi 0.00223

MarkerSeek score 60.10

Primer available yes

Label trnR-ACG-trnN-GTT

Feature Metadata

label_name: trnR-ACG-trnN-GTT
feature_type: igs
region: IRb
length_bp: 597
variable_sites: 2
pi: 0.00223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GTT_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	774-788 bp	1.0	79.6
trnR-ACG-trnN-GTT_p2	AACAGATCCAGTGGAGACGA	TGGGTTGAGAACGGGAATTG	50.0	50.0	58.1	58.1	815-829 bp	1.0	78.8
trnR-ACG-trnN-GTT_p3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	738-752 bp	1.0	78.5
trnR-ACG-trnN-GTT_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	738-752 bp	1.0	78.4
trnR-ACG-trnN-GTT_p5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	725-739 bp	1.0	78.4