Hotspot

trnA-TGC-rrn23S

IRb · 109808-109979 · 172 bp

Back to Theobroma

Pi 0.00388
MarkerSeek score 60.60
Primer available yes
Label trnA-TGC-rrn23S

Feature Metadata

label_name
trnA-TGC-rrn23S
feature_type
igs
region
IRb
length_bp
172
variable_sites
1
pi
0.00388
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-TGC-rrn23S_p1 GGTTCGAGTCCGCTTATCTC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 230-280 bp 1.0 81.8
trnA-TGC-rrn23S_p2 GTTCGAGTCCGCTTATCTCC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 229-279 bp 1.0 81.8
trnA-TGC-rrn23S_p3 GGTTCGAGTCCGCTTATCTC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 302-352 bp 1.0 81.6
trnA-TGC-rrn23S_p4 GTTCGAGTCCGCTTATCTCC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 301-351 bp 1.0 81.6
trnA-TGC-rrn23S_p5 TTCGAGTCCGCTTATCTCCA ATTCGGGAATCTCCGGATCT 50.0 50.0 58.2 58.0 300-350 bp 1.0 80.7