Hotspot

rps19-rpl2

IRb · 89228-89311 · 84 bp

Back to Theobroma

Pi 0.0238

MarkerSeek score 67.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 84
variable_sites: 3
pi: 0.0238
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	229-230 bp	1.0	79.9
rps19-rpl2_p2	ATGGTAGATGCTCTGGACCA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.8	58.1	230-231 bp	1.0	78.8
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	287-288 bp	1.0	78.7
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.0	58.3	231-232 bp	1.0	78.4
rps19-rpl2_p5	ATGGTAGATGCTCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	288-289 bp	1.0	77.7