Hotspot

rbcL-accD

LSC · 60679-61399 · 721 bp

Back to Theobroma

Pi 0.00462

MarkerSeek score 61.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 5
pi: 0.00462
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CGGGTCTTCGCTTACACTAG	55.0	55.0	58.0	57.8	936-937 bp	1.0	78.7
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	GCCCAAGGCTATCCATTGAT	55.0	50.0	58.0	57.7	904-905 bp	1.0	77.8
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	CGGGTCTTCGCTTACACTAG	55.0	55.0	57.7	57.8	922-923 bp	1.0	76.7
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	CGGGTCTTCGCTTACACTAG	55.0	55.0	58.3	57.8	934-935 bp	1.0	76.4
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GCCCAAGGCTATCCATTGAT	55.0	50.0	57.7	57.7	890-891 bp	1.0	75.8