Hotspot

atpF-atpH

LSC · 13256-13863 · 608 bp

Back to Theobroma

Pi 0.00566

MarkerSeek score 65.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 5
pi: 0.00566
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGGTTGGTTGTAGCATTGG	50.0	50.0	58.0	58.4	686-698 bp	1.0	79.5
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	GCATTGGCGCTTTTATTTGC	50.0	45.0	58.0	57.6	673-685 bp	1.0	79.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGGTTGGTTGTAGCATTGG	50.0	50.0	57.9	58.4	714-726 bp	1.0	78.7
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCATTGGCGCTTTTATTTGC	50.0	45.0	57.9	57.6	701-713 bp	1.0	78.2
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.0	57.3	756-768 bp	1.0	76.5