Hotspot

trnR-TCT-atpA

LSC · 10156-10279 · 124 bp

Back to Theobroma

Pi 0.0376

MarkerSeek score 61.40

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 7
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACTGAGGAAGCGGAA	47.6	50.0	57.3	57.9	226-248 bp	1.0	68.0
trnR-TCT-atpA_p2	GGTTCAAATCCTATTGGACGC	CTGAGGAAGCGGAAACACTT	47.6	50.0	57.3	58.1	220-242 bp	1.0	67.9
trnR-TCT-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCACTGAGGAAGCGGAAAC	47.6	50.0	57.3	58.1	224-246 bp	1.0	67.8
trnR-TCT-atpA_p4	GGTTCAAATCCTATTGGACGC	TGAGGAAGCGGAAACACTTT	47.6	45.0	57.3	57.7	219-241 bp	1.0	66.1
trnR-TCT-atpA_p5	GGTTCAAATCCTATTGGACGC	AGACATTCACTGAGGAAGCG	47.6	50.0	57.3	57.6	229-251 bp	1.0	65.7