Hotspot

trnK-TTT-rps16

LSC · 4497-5302 · 806 bp

Back to Theobroma

Pi 0.00838

MarkerSeek score 65.20

Primer available yes

Label trnK-TTT-rps16

Feature Metadata

label_name: trnK-TTT-rps16
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 10
pi: 0.00838
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-TTT-rps16_p1	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	933-943 bp	1.0	80.3
trnK-TTT-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	934-944 bp	1.0	80.2
trnK-TTT-rps16_p3	CCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.3	58.0	943-953 bp	1.0	79.3
trnK-TTT-rps16_p4	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	920-930 bp	1.0	77.9
trnK-TTT-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	893-903 bp	1.0	77.4