Hotspot

rpl14-rpl16

LSC · 77815-77926 · 112 bp

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Pi 0.0137
MarkerSeek score 55.30
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
112
variable_sites
5
pi
0.0137
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 GATTACCAGCGGCTCCTATG TAGCTGCCAGTAAAATGCCC 55.0 50.0 57.9 58.2 255-257 bp 1.0 79.6
rpl14-rpl16_p2 ATTACCAGCGGCTCCTATGA TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.3 58.2 254-256 bp 1.0 78.0
rpl14-rpl16_p3 TGATTACCAGCGGCTCCTAT TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.3 58.2 256-258 bp 1.0 78.0
rpl14-rpl16_p4 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 55.0 50.0 58.4 58.2 214-216 bp 1.0 77.2
rpl14-rpl16_p5 GATTACCAGCGGCTCCTATG GCCAGTAAAATGCCCATACG 55.0 50.0 57.9 57.2 250-252 bp 1.0 75.3