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psbE-petL

LSC · 62503-63765 · 1263 bp

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Pi 0.0076

MarkerSeek score 54.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 29
pi: 0.0076
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1353-1429 bp	1.0	81.7
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1351-1427 bp	1.0	81.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1342-1418 bp	1.0	81.4
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1340-1416 bp	1.0	81.4
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.2	58.5	1344-1420 bp	1.0	79.9