Hotspot

psaA-ycf3

LSC · 42016-42630 · 615 bp

Back to Themeda

Pi 0.00905

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 14
pi: 0.00905
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	834-932 bp	1.0	80.4
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	799-897 bp	1.0	80.0
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.0	57.6	801-899 bp	1.0	79.8
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCTATAGCGCTTACTCCAGG	50.0	55.0	58.1	57.6	766-864 bp	1.0	79.5
psaA-ycf3_p5	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	737-835 bp	1.0	79.1