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atpI-atpH

LSC · 31915-32741 · 827 bp

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Pi 0.00639

MarkerSeek score 66.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 16
pi: 0.00639
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	CAGTACCTTGACCAACTCCG	50.0	55.0	57.5	57.9	878-962 bp	1.0	80.5
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	CAGTACCTTGACCAACTCCG	45.0	55.0	58.6	57.9	891-975 bp	1.0	80.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	879-963 bp	1.0	79.6
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	812-896 bp	1.0	79.6
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	834-918 bp	1.0	79.5