Hotspot

trnT-GGU-trnE-UUC

LSC · 15100-15568 · 469 bp

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Pi 0.0325

MarkerSeek score 75.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 34
pi: 0.0325
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.4	58.3	497-618 bp	1.0	81.9
trnT-GGU-trnE-UUC_p2	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	50.0	50.0	57.4	57.7	476-597 bp	1.0	81.9
trnT-GGU-trnE-UUC_p3	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	50.0	55.0	57.4	57.4	473-594 bp	1.0	79.5
trnT-GGU-trnE-UUC_p4	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	50.0	55.0	57.4	57.4	472-593 bp	1.0	79.5
trnT-GGU-trnE-UUC_p5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	58.1	58.3	497-618 bp	1.0	78.5