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psbZ-trnG-GCC

LSC · 11998-12281 · 284 bp

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Pi 0.00623

MarkerSeek score 35.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 6
pi: 0.00623
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	450-451 bp	1.0	76.3
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	448-449 bp	1.0	75.9
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TTACCACTGAACTACGCTCG	50.0	50.0	57.8	57.4	415-416 bp	1.0	74.9
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	TTACCACTGAACTACGCTCG	55.0	50.0	58.3	57.4	413-414 bp	1.0	74.5
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	CCGTATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.3	444-445 bp	1.0	74.3