Hotspot

rpl23-trnI-CAU

LSC · 82293-82472 · 180 bp

Back to Thelypteris

Pi 0.0296
MarkerSeek score 61.70
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
LSC
length_bp
180
variable_sites
8
pi
0.0296
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 GCTGCAACAAACGAATAGACT TGGCGAATTCACAGGTTCAA 42.9 45.0 57.2 58.0 258-263 bp 1.0 68.7
rpl23-trnI-CAU_p2 GCTGCAACAAACGAATAGACT TTGGCGAATTCACAGGTTCA 42.9 45.0 57.2 58.0 259-264 bp 1.0 68.6
rpl23-trnI-CAU_p3 GCTGCAACAAACGAATAGACT GTTTGCATCCATGGCTGAAC 42.9 50.0 57.2 58.0 301-306 bp 1.0 68.4
rpl23-trnI-CAU_p4 GCTGCAACAAACGAATAGACT CATCCATGGCTGAACGGTTA 42.9 50.0 57.2 58.0 296-301 bp 1.0 68.1
rpl23-trnI-CAU_p5 GCTGCAACAAACGAATAGACT TGAACGGTTAAAGCACCCAA 42.9 45.0 57.2 57.9 286-291 bp 1.0 68.1