Hotspot

clpP-psbB

LSC · 68304-68833 · 530 bp

Back to Thelypteris

Pi 0.0151

MarkerSeek score 61.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 12
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CCTTTGGAACACCAATTGGC	ACGAACATACCCTGTCTCCA	50.0	50.0	57.8	58.1	665-666 bp	1.0	79.6
clpP-psbB_p2	CACTATACGTCAACCCGAGC	ACGAACATACCCTGTCTCCA	55.0	50.0	58.2	58.1	717-718 bp	1.0	79.5
clpP-psbB_p3	GCACTATACGTCAACCCGAG	ACGAACATACCCTGTCTCCA	55.0	50.0	58.2	58.1	718-719 bp	1.0	79.5
clpP-psbB_p4	CACTATACGTCAACCCGAGC	GGGATCCGATGGGTCAAAAA	55.0	50.0	58.2	57.9	682-683 bp	1.0	79.0
clpP-psbB_p5	GCACTATACGTCAACCCGAG	GGGATCCGATGGGTCAAAAA	55.0	50.0	58.2	57.9	683-684 bp	1.0	79.0