Hotspot

cemA-psbJ

LSC · 58692-60730 · 2039 bp

Back to Thelypteris

Pi 0.015

MarkerSeek score 67.80

Primer available yes

Label cemA-psbJ

Feature Metadata

label_name: cemA-psbJ
feature_type: igs
region: LSC
length_bp: 2039
variable_sites: 46
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-psbJ_p1	GCCTTGTCTCAACCTTTCCA	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	2227-2236 bp	1.0	81.3
cemA-psbJ_p2	TGCCTTGTCTCAACCTTTCC	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	2228-2237 bp	1.0	81.3
cemA-psbJ_p3	TCGCACATCTCCCTCAATTG	GCCGGTACACTTGTGATAGG	50.0	55.0	58.0	58.1	2160-2169 bp	1.0	81.3
cemA-psbJ_p4	GCCTTGTCTCAACCTTTCCA	TCCTCTTACGGGGCTTACTC	50.0	55.0	58.0	58.2	2194-2203 bp	1.0	80.1
cemA-psbJ_p5	TGCCTTGTCTCAACCTTTCC	TCCTCTTACGGGGCTTACTC	50.0	55.0	58.0	58.2	2195-2204 bp	1.0	80.1