Hotspot

trnF-GAA-ndhJ

LSC · 46501-46727 · 227 bp

Back to Thelypteris

Pi 0.0294

MarkerSeek score 64.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 10
pi: 0.0294
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTGATGCCTGAAAGTTGGGT	50.0	45.0	58.1	57.6	345 bp	1.0	78.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGATGCCTGAAAGTTGGGTG	50.0	50.0	58.1	58.4	344 bp	1.0	78.9
trnF-GAA-ndhJ_p3	CCGAGATAGCTCAGTCGGTA	TTGATGCCTGAAAGTTGGGT	55.0	45.0	58.1	57.6	383 bp	1.0	78.1
trnF-GAA-ndhJ_p4	CCGAGATAGCTCAGTCGGTA	TGATGCCTGAAAGTTGGGTG	55.0	50.0	58.1	58.4	382 bp	1.0	78.1
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	TTGATGCCTGAAAGTTGGGT	50.0	45.0	57.5	57.6	348 bp	1.0	75.6