Hotspot

trnC-GCA-trnG-GCC

LSC · 29842-30286 · 445 bp

Back to Thelypteris

Pi 0.0232

MarkerSeek score 68.40

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 15
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	487-505 bp	1.0	76.1
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	488-506 bp	1.0	75.9
trnC-GCA-trnG-GCC_p3	TTAGGCGACACCCAGATTTG	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	522-540 bp	1.0	75.6
trnC-GCA-trnG-GCC_p4	GTTAGGCGACACCCAGATTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	523-541 bp	1.0	75.6
trnC-GCA-trnG-GCC_p5	TTTCTTGTTAGGCGACACCC	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	529-547 bp	1.0	75.5